Fig. S6. Mesodermal competence is regulated by interplay between nucleosomal H3 and linker histone H1. (A) Synthesis of somatic linker histone H1A is inhibited by H1A antisense MO. Western blot analysis of acid nuclear extracts from gastrula stage control or H1A MO-injected embryos shows ~70% knockdown of histone H1 (arrow). (B) Western blot analysis of endogenous H3 protein levels in gastrula stage control and injected embryos. (C) Time-lapse images tracking development of control and H1A-depleted embryos. Development of H1A knockdown embryos was slightly delayed but otherwise normal. (D) Percentage of control and injected embryos that express Xbra. Embryos from two independent experiments were fixed at early gastrula and subjected to RNA in situ hybridization analysis of Xbra transcripts. (E) Protein synthesis of injected mRNAs shown by western blot analysis of cellular extracts from stage 10.5 embryos.
Image published in: Lim CY et al. (2013)
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