Fig. 1. Presence of CREB-like protein and CREB mRNA in Xenopus embryos. (A) Bandshift experiments using 32P-labeled CRE oligonucleotides and stage 20 embryonic nuclear extracts show the presence of CRE-binding activity (lanes 1 and 2). Anti-CREB antibodies (lanes 3 and 4) and anti-phospho-CREB antibodies (lane 5) induced strong supershifts, but the antiserum ATF1.1 which is speci®c for ATF1 was unable to produce a supershift (lane 6). Antiserum ATF1.2 recognizing both CREB and ATF1 produced a strong supershift (lane 8). Adding a 100-fold excess of unlabeled CRE oligonucleotides completely abolished bandshift signal (lane 9). (B) Northern blot analysis showing a speci®c 2.6 kb band representing Xenopus CREB mRNA.
Image published in: Lutz B et al. (1999)
Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-79989