Figure 2. Downregulation of transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-C) The expression of sox2 was not changed in all three conditions. (D-O) The expression of nscl-1 (E,F), neurod (H,I), aml1 (K,L), and emx1 (N,O) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (D,G,J,M). Panels (A-L) show dorsal views of neurula stage embryos (stage 15/16), and (M-O) are anterior views of tail bud stage embryos (stages 25 to 28).
Image published in: Green YS and Vetter ML (2011)
Copyright ©2011 Green and Vetter; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nhlh1.L | bhlha35, hen-1, hen1, nhlh1-a, nhlh1-b, nscl, nscl1 | X. laevis | Throughout NF stage 15 | neuron Rohon-Beard neuron trigeminal placode |
| runx1.L | aml, aml-1, aml1, aml1 oncogene, aml1-evi-1, amlcr1, cbfa2, evi-1, LOC108709238, pebp2ab, Runx-1, Xaml, Xaml1 | X. laevis | Throughout NF stage 15 | neuron chordal neural plate border |
| emx1.S | empty spiracles homolog 1 | X. laevis | Sometime during NF stage 25 to NF stage 28 | forebrain |
| neurod1.L | neurod, neuroD, neurod1-a, neurod1-b, XNeuroD | X. laevis | Throughout NF stage 15 | neuron trigeminal placode chordal neural plate border |
| sox2.L | anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 | X. laevis | Throughout NF stage 15 | brain forebrain midbrain hindbrain neural plate midbrain-hindbrain boundary pre-chordal neural plate chordal neural plate anterior neural ridge anterior neural fold |
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