Figure 4. 5′ sequence of SPC7. (A) 5′ sequence of SPC7 and antisense morpholino oligonucleotides. Sequences corresponding to SPC7 MOs 1 and 2 are shown in green and magenta, respectively. Start codon is underlined. Arrow denotes 5′ primer used to construct synthetic mRNA used in rescue experiments. (B) Sequences of antisense morpholino oligonucleotides. (C, D) Bright and dark field images of stage 37 SPC7 antisense morpholino-only control embryo showing anophthalmic SPC7-MO phenotype on injected side. (E) Histological section through SPC7-MO embryo; note lack of distinguishable eye structures on the injected side and severe mesencephalic hypoplasia. (F, G) Bright and dark field images of stage 37 embryo coinjected with SPC7 antisense morpholino oligonucleotides and synthetic rescue mRNA, showing normal morphology on the injected side. For all rescue experiments, we used the 90 ng dose. Complete rescue as shown in (F-H) was observed in all embryos in each of three separate experiments (106 total embryos were scored); identical results were obtained with either SPC7-MO. (H) Histological section through coinjected embryo; both eyes appear normal.
Image published in: Senturker S et al. (2012)
Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Permanent Image Page
Printer Friendly View
XB-IMG-80196