FIG. 3. Isolated cDNA clones expressed in the AEM region and notochord. (A) Clone names and similar genes or proteins in the public databases are indicated on the left side of each panel. Results of whole-mount in situ hybridization for early neurula (stage 13) are shown in dorsal (left) and lateral (right) view. (B) Northern blot analysis. Clone names and their closest genes or proteins in the public databases are indicated on the left side of each panel and the deduced mRNA size (kb) of each clone on the right (see Table 2 for P11F3). The developmental stages are indicated on the top of each column by numerical figures according to Nieuwkoop and Faber (1967). 18S rRNA stained with ethidium bromide is for loading control. Ten micrograms of total RNA from stage-9, -10, -12.5, and -15 embryos were electrophoresed in each lane, blotted, and hybridized with probes derived from each clone except for crescent. Crescent probe was hybridized to the same blot as previously used (Hikasa and Taira, 2001) with a wider range of stages as indicated.
Image published in: Shibata M et al. (2001)
Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| spty2d1.S | X. laevis | Throughout NF stage 13 | axial mesoderm prechordal plate dorsal | |
| gpr107.L | X. laevis | Throughout NF stage 13 | axial mesoderm prechordal plate dorsal involuted dorsal mesoderm | |
| syngr1.L | synaptogyrin, synaptogyrin 1, synaptogyrin-1 | X. laevis | Throughout NF stage 13 | axial mesoderm anterior dorsal |
| btbd6.L | xbtbd6 | X. laevis | Throughout NF stage 13 | axial mesoderm anterior dorsal anterior neural ridge |
| ostc.L | dc2, ostc-a, ostc-b | X. laevis | Throughout NF stage 13 | axial mesoderm prechordal plate dorsal |
| mob2.L | hcca2, LOC108715206, mob2.1 | X. laevis | Throughout NF stage 13 | axial mesoderm dorsal involuted dorsal mesoderm |
| tmem52.L | X. laevis | Throughout NF stage 13 | axial mesoderm prechordal plate anterior dorsal neural plate border | |
| nudt22.L | X. laevis | Throughout NF stage 13 | endomesoderm prechordal plate anterior mesoderm involuted dorsal mesoderm |
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