Figure 6. Absence of lhx1 affects all the domains of the pronephric kidney.Embryos were injected (1xV2) with 300 pg of lhx1-AS at the 8-cell stage. (A–O) In situ hybridization of embryos at stage 32. (A–C) In situ hybridization for follistatin (fst). (A) Uninjected embryo. (B, C) Control and injected sides of the same embryo are shown. (C) Reduced fst expression was observed in 57% of the embryos (n = 44). (D–F) In situ hybridization for pax2. (D) Uninjected embryo. (E, F) Control and injected sides of the same embryo are shown. (F) Reduced pax2 expression was observed in 86% of the embryos (n = 32). (G–I) In situ hybridization for hoxb7. (G) Uninjected embryo. (H, I) Control and injected sides of the same embryo are shown. (I) Reduced hoxb7 expression was observed in 92% of the embryos (n = 38). (J–L) In situ hybridization for laminin-β1. (J) Uninjected embryo. (K, L) Control and injected sides of the same embryo are shown. (L) Reduced laminin-β1 expression was observed in 94% of the embryos (n = 32). (M–O) In situ hybridization for neuropilin1 (nrp1). (M) Uninjected embryo. (N, O) Control and injected sides of the same embryo are shown. (O) Reduced nrp1 expression was observed in 90% of the embryos (n = 40). (P–U) In situ hybridization of embryos at stage 24 for wt1. (P, Q) Uninjected embryo. (R–U) Control and injected sides of the same embryo are shown. (T, U) Reduced wt1 expression was observed in 89% of the embryos (n = 37). (Q, S, U) Transverse sections of embryos in P and R/T, respectively. Arrows indicate plane of section in appropriate panels.
Image published in: Cirio MC et al. (2011)
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| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| fst.L | foll, follistatin, xfs | X. laevis | Throughout NF stage 32 | pronephric kidney visceral pouch |
| pax2.L | LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 | X. laevis | Throughout NF stage 32 | pronephric tubule early distal tubule early proximal tubule otic vesicle pronephric kidney optic stalk hindbrain pronephric nephrostome spinal cord |
| hoxb7.S | hho.c1, hox-2.3, hox2, hox2c, hoxb7-a, hoxb7-b, MM3, xeb2, Xhox45, XlHbox2 | X. laevis | Throughout NF stage 32 | spinal cord tail tip pronephric duct |
| lamb1.S | clm | X. laevis | Throughout NF stage 32 | otic vesicle midbrain-hindbrain boundary midbrain pineal gland intersomitic region tail tip visceral pouch pronephric duct pronephric tubule early distal tubule early proximal tubule head region head vasculature |
| nrp1.L | A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 | X. laevis | Throughout NF stage 32 | head region pronephric duct |
| wt1.S | wt-1, wt1-a, wt1-b, Xwt-1, xwt1 | X. laevis | Throughout NF stage 24 | pronephric kidney glomus |
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