Fig. S3. Validation of Tcf21-MO specificity. (A) Schematic showing relative MO positioning on Xenopus Tcf21 mRNA; numbers denote nucleotide base pair positions. Validation of MO-specific Tcf21 translation inhibition by V5 western blot on stage 12 embryos; representative blot from four independent experiments. Shp2 protein provided a loading control, in vitro translated Tcf21-V5 a positive control. Molecular weight standards are included to right of blot.
Image published in: Tandon P et al. (2013)
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