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Figure 8. Validation of microarray data. Following 4 h of Ngn3-GR activation at Stage 12 candidate genes from the microarray analysis were increased in the endoderm at Stage 15: (a,b) tbx2 (n = 24/24). (c,d) mtg8 (n = 21/21). (e,f) hes3 (n = 14/42). (g,h) geminin (n = 13/44). (i,j) oct25 (n = 13/13). And at Stage 20: (k,l) mtgr1 (n = 9/13). Dashed circles and arrows indicate ectopic expression in the anterior endoderm. Dashed line indicates ectopic expression in the dorsal endoderm.

Image published in: Oropeza D and Horb M (2012)

Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.

tbx2.LXltbx2, xtbx2, ET, tbx2-a, tbx2-bX.laevisThroughout NF stage 15involuted ventral mesoderm
cement gland primordium
preplacodal ectoderm
runx1t1.LMTG8X.laevisThroughout NF stage 15foregut
pre-chordal neural plate
presumptive midbrain-hindbrain boundary
prechordal plate
hes5.10.Lesr6e, esr-6eX.laevisThroughout NF stage 15foregut
circumblastoporal collar
involuted dorsal mesoderm
involuted ventral mesoderm
suprablastoporal endoderm
gastrocoel roof plate
gmnn.Sgeminin, gemX.laevisThroughout NF stage 15neural plate
pre-chordal neural plate
chordal neural plate
pou5f3.2.Lxoct-25, Oct-25, xoct25, oct25, pou25X.laevisThroughout NF stage 15chordal neural plate
cbfa2t2.LMTGR1, XETORX.laevisThroughout NF stage 15liver diverticulum
chordal neural plate
neural plate
pre-chordal neural plate

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