Fig. 3. PACSIN2 over-expression inhibits fibronectin fibrillogenesis. Embryos were injected in both blastomeres at the two-cell stage. The blastocoel roofs (BCR) were dissected at stage 12, fixed and processed for immunofluorescence. The FN fibrils of the BCR were detected using a rabbit polyclonal antibody 32F (red) while PACSIN2 was detected with the mAb 3D8 (green). The region indicated by white arrows is magnified in the merge (4 inset. While all photographs were taken with the same settings, the endogenous PACSIN2 detection (4 left) has been enhanced using Photoshop in the magnified image of NI cell to visualize the cell borders. Over-expression of Wt PACSIN2 in the entire BCR prevents FN fibrillogenesis. The expression of ∂CC PACSIN2 allows the BCR cells to assemble some FN fibrils.
Image published in: Cousin H et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| pacsin2.S | sdpii, x-PACSIN2 | X. laevis | Throughout NF stage 12 | blastocoel roof |
| fn1.S | fibronectin, gene C, LOC121393057, MGC97508 | X. laevis | Throughout NF stage 12 | blastocoel roof |
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