Fig. 5. Mixed Up. (A) Control wild-type embryo where the conotruncus of the heart loops to the left from the right anterior aspect of the ventricle and the gut has a right coil origin (RO) and counter-clockwise coils (CCW). (B and C) Mixed-Up mutants displaying abnormal heart and gut looping. The mutant tadpole in (B) exhibits a left coil origin (LO) for the gut whereas the mutant tadpole in (C) exhibits a right coil origin (RO) for the gut. Both mutants have clockwise coils (CW). (D and E) Mixed-Up mutant tadpoles may display alterations in gene expression at earlier stages of development. (D) illustrates the right hand view and E the left-hand view of three mutant tadpoles that are stained for Nkx2.5 expression. The tadpole at the top of (D) and (E) displays an altered expression of Nkx2.5 in the developing gut (D, arrowhead) whereas normal gut expression is observed in its two mutant siblings below it (E, arrowheads). This altered expression is indicative of the fact that this tadpole has an asymmetry defect affecting the direction of gut looping.
Image published in: Noramly S et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-81989