Fig. 7. Alternative splicing of XPax-2 transcripts in the developing pronephric kidney. (A) Outline of the dissection strategy. To illustrate the distribution of XPax-2 expressing tissues, embryos of stage 24 and 36, respectively, stained by in situ hybridization are shown. The dissection planes are indicated with black lines. Heads (h) and explants enriched in pronephric tissue (p) were used for RNA isolation, and subsequent RT-PCR analysis. Head explants contain brain tissues, sensory organs, and visceral arch material. (B) Identification of alternative splice variants using primer pair XP-59/XP-63. Head (h) and pronephric (p) explants were analyzed by RT-PCR. The autoradiograph is shown with the size markers on the left. Amplification products verified by DNA sequencing are identified by arrowheads and their exon compositions are shown. (C) Identification of alternative splice variants using primer pair XP-9/XP- 24. (D) Control for equal RNA amounts. RT-PCR experiments were carried out in parallel with histone H4 specific primer pair H4-1/H4-2. Amplification products were separated by agarose gel electrophoresis and visualized by staining with ethidium bromide. M, lane with size markers. The limitations of histone H4 as a control for pronephric RNA preparations are discussed in Section 2.7.
Image published in: Heller N and Brändli AW (1997)
Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V.
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