Fig. 7. Analysis of effects of Xmab21l3 on BMP and FGF/ERK signaling. (A) Injection of BMP2 RNA inhibits neuralization by Xmab21l3. Graphs show relative expression, assayed by RT-PCR, of the neural markers sox2 and sox3 in animal caps normalized to expression in uninjected explants. (B) Xmab21l3 misexpression inhibits expression of the BMP-responsive gene sizzled (szl), and induces expression of the dorsal marker fgf4. (C) Xmab21l3 misexpression inhibits expression of a Vent2-Luciferase reporter fusion protein; this construct includes a mutation in a TCF binding site that renders it insensitive to Wnt activation (Hikasa et al., 2010). (D) Xmab21l3 activates ERK1/2 signaling in animal cap explants. Western blot analysis using an antibody against di-phosphorylated ERK1/2 demonstrates that ERK1/2 is activated in response to treatment with FGF or following injection of Xmab21l3 RNA. The FGFR1 inhibitor SU5402 blocks ERK activation by FGF, Xmab21l3 and Xmab21l3SMT. Error bars denote s.d.
Image published in: Sridharan J et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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