Figure 6. Epistasis Experiments Suggest that BMP Acts Upstream of Notch in Tail Regeneration (A-D) Effect of a Notch inhibitor on BMP pathway-driven refractory stage regeneration. Tadpoles were amputated at stage 47 and given daily heat shocks for 1 week. (A) Wild-type (WT) tadpoles do not regenerate at this stage.(B) Sibling tadpoles expressing the HSP70-Alk3-γCrys-GFP regenerate all the tail tissues.(C and D) Neither WT (C) nor transgenic tadpoles (D) will regenerate any tail tissue following treatment with 10 μM Notch proteolysis inhibitor MG132.(E and F) Effect of combined inhibition of BMP signaling and activation of the Notch pathway. The tadpoles express both HSP70-tBr-γCrys-GFP and HSP70-NICD-γCrys-RFP. Following amputation after stage 50, when WT animals regenerate tails normally, these double transgenics regenerate only notochord and spinal cord tissue. No new muscle is formed. White arrowheads mark the site of amputation in (B, E, and F).
Image published in: Beck CW et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
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