Fig. 2. Interfering with proximal tubular growth caused a defect in yolk platelet degradation. (A to B″) Xenopus embryos were treated with 2 μg/ml Ctx at stage 29/30 and analyzed at stage 42 using transmission electron microscopy at a 2650-fold (A and B) or 31,000-fold magnification (A′, A″, B′ and B″). Position of the insets is indicated by the black boxes in (A and B). Arrows indicate the gaps between the proximal tubular cells upon Ctx treatment, arrowheads point towards the membrane layer surrounding the degrading yolk platelets. (C and D) PAS staining to visualize protein-rich granules in the proximal tubules. Inset shows close-up of a single cell. (E and H) Immunofluorescence staining of proximal tubules using 3G8 (red), anti-vitellogenin or anti-seryp antibodies (green). (E′ and F′) are close-ups of the proximal tubules indicated by white boxes in (E and F); the asterisks indicate the distal tubules; nuclei were counterstained with DAPI (blue). (I and J) Quantification of anti-vitellogenin staining for untreated and Ctx treated embryos at stage 35 and 40 (I) as well as untreated and treated with Hydroxyurea/Aphidicolin (HUA) at stage 40 (J).
Image published in: Zhang B et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-83411