Figure S5. RAB8B Splicing Morpholino Reduces Wnt Activity in Tg(7xTCF-XLa.Siam:eGFP)ia4 Zebrafish Embryos, Confirmation of Its Specificity, Related to Figure 5 (A) Dorsal and lateral views of rab8b splicing morpholino (MoSpl) injected Tg(7xTCF-XLa.Siam:eGFP)ia4 zebrafish embryos with reduced GFP intensity in MHB and OV (n = 26/50). (B) RT-PCR analysis of zebrafish embryos (at 30hpf) injected with rab8b MoSpl revealed higher levels of unspliced rab8b transcript, while the amount of control transcripts, wnt7aa and runx1t1, remained unchanged.
Image published in: Demir K et al. (2013)
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