Fig. 2. Dose-dependent defects in cardiac morphogenesis caused by the retinoic acid antagonist. To better visualize the defects in cardiac morphogenesis due to addition of antagonist, αMHC expression was examined in serial sections along the anterior–posterior axis of stage 32/34 embryos subjected to varying amounts of AGN 194301. A complete tube with distinct left–right asymmetry was clearly evident throughout most of the control myocardial domain (first column, A, E, I, M, Q with anterior at the top and posterior at the bottom). Consistent with the whole-mount data, no evidence of linear heart tube formation was detected in 1 μM AGN 194301 treatments (second column, B, F, J, N, R), while a partial, relatively symmetrical heart tube could be detected within 100-nM-treated embryos (third column, C, G, K, O, S). Formation of a heart tube was observed in embryos subjected to 10 nM AGN 194301 (fourth column, D, H, L, P, T), although the fusion was not as extensive as seen in control embryos. In addition, at this stage, the left and right side appeared symmetric when compared to control embryos.
Image published in: Collop AH et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-83513