Figure 6. In vitro induction of pronephros or pancreas from animal caps treated with activin and retinoic acid (RA). Animal caps were excised and treated with activin and/or RA in Steinberg's solution (SS) supplemented with 0.1% bovine serum albumin (BSA). Explants were washed with SS and cultured in SS with 0.1% BSA. All the treatments and culturing were performed at 20°C. A: When animal caps were excised and treated with 10 ng/ml activin and 104 M RA simultaneously for 3 hr, washed and cultured for 4 days. The explants mainly differentiated into pronephric tissues. After this explant was transplanted to a pronephrectomized Xenopus embryo of stage 20–25, the transplanted embryo developed normally. In contrast, the pronephrectomized Xenopus embryo showed edema at the tadpole stages. B: When animal caps were treated with 100 ng/ml activin for 1 hr, washed and cultured for 3–5 hr in SS with 0.1% BSA, and then treated with 104 M RA for 1 hr, the explants differentiated into pancreatic tissues at a high frequency.Download figure to PowerPoint
Image published in: Asashima M et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.
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