Figure 1. Glycosylation of Lefty molecules. A,B: Medium was analyzed from Xenopus oocytes injected with the indicated RNAs, metabolically labeled with [35S]-methionine/cysteine. Black dots, major glycosylated and deglycosylated bands. C: Western blot of conditioned medium from dissociated animal halves isolated from embryos injected at the one-cell stage with 2.5 ng of XleftyHA RNA. Un, uninjected oocytes or embryos; V, vehicle (1% DMSO); (−), oocytes not injected with tunicamycin, or conditioned medium not treated with PNGase F; T, tunicamycin; P, PNGase F; L, XleftyL (filled arrowhead); S, XleftyS (open arrowhead).Download figure to PowerPoint
Image published in: Westmoreland JJ et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons.
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