Fig. 8. Transverse sections of stage-35 embryos stained for vimentin. In a transverse section of the head stained with 14h7 (A), vimentin is found in most of the head mesenchyme (hm) surrounding the brain, in the meninges (inn), in the corneal endothelium (ce) and in the mandibular arch (ma). There is also some mesenchyme (em) in the eye. Vimentin is expressed in cells of the mesencephalon and diencephalon (nv). These cells exhibit the characteristic morphology of mature radial glia, i.e. they are very thin and extend from the margin to the ventricular surface. The staining of the ectoderm is artifactual. A transverse section through an anterior somite of the dorsal trunk (B) reveals that vimentin is expressed in the dorsal fin mesenchyme (dm). Vimentin is also found in the mesenchyme associated with the outer surface of the somites (sm) and in mesoderm below the pronephric ducts (pm). Vimentin-expressing cells are present in the neural tube and extend from the pial to the ventricular surface (nv). In the area of the embryo ventral to the region illustrated in B (C) are found the lateral body wall and the yolky endoderm ventral to the pronephros. The pronephric rriesoderm is at the top (pm) and below it, between the mesoderm and the endoderm are the vitelline veins (vv) which contain vimentin-expressing mesenchyme. c, cement gland; de, diencephalon; df, dorsal fin; e, eye; me, mesencephalon; n, notochord; nt, neural tube (spinal chord); p, pharynx; pd, pronephric duct; s, somite; vv, vitelline veins. Bars, 50/.im in A & B, 25 ^m in C
Image published in: Dent JA et al. (1989)
Copyright © 1989. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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