Figure 2. Loss-of-functional effects by antisense morpholino oligomer for Adrβ2 (Adrβ2-MO) for Xenopus embryogenesis. (A) Experimental design using Adrβ2-MO. Adrβ2-MO was designed to target the translation initiation site (blue bar). This MO does theoretically not bind to artificially created mRNA containing five mismatched nucleotides (5mis) shown below. Red and green letters indicate start codon and changed nucleotides, respectively, without changing coding information. (B) Control embryo at stage 38. (C) Adrβ2-MO injected embryos. 75% (6/8) of embryos lost tail structures. (D) Adrβ2 mRNA injected embryos. 89.5% (17/19) of embryos lost eye structures. (E) 5mis mRNA injected embryos.88.2% (15/17) of them lost eye structures. (F) Adrβ2-MO and Adrβ2 mRNA coinjected embryos. 9.5% (2/21) of embryos lost tail structures. (G) Adrβ2-MO and 5mis mRNA coinjected embryos. No embryo lost tail structures, and 53.6% (15/28) of embryos looked almost normal. Scale bars represent 1 mm.
Image published in: Mori S et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons.
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