Figure 5. Rspo2 Promotes Myogenesis in Xenopus Embryos (A) Diagram of the experiments. 4-cell stage embryos were injected with 50 pg plasmid DNA constructs in all blastomeres, and the indicated fragments were explanted at stage 10.5, cultured, and processed for whole-mount in situ hybridization (C) or RT-PCR (D). (B) Stage 40 equivalent VMZ or LMZ explants. Note tail-like structures in VMZs from Rspo2-injected embryos. (C) In situ hybridization of stage 25 VMZs for muscle actin. (D) RT-PCR analysis for the indicated genes in stage 11 equivalent DMZ and stage 25 equivalent VMZ explants. Xdd1, dominant-negative Xenopus dishevelled, Co, preprolactin. −RT, minus reverse transcription control.
Image published in: Kazanskaya O et al. (2004)
Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.
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