Figure S1, related to Figures 1-4 and 6. Morpholino design and general phenotype of Tel1 morphants (A) Two antisense MO oligonucleotides were designed to block the translation of X. laevis Tel1. MO1 (pink) targets a sequence overlapping with the translation initiation codon whereas MO2 (red) targets a sequence within the 5� UTR. The targets of both MO1 and MO2 are conserved between the two pseudoallelic forms of Tel1 found in the X. laevis genome. (B) Structure of Tel1-GFP fusion construct used to test the activity of Tel1 MO1. (C) Embryo injected with the Tel1-GFP fusion mRNA shows GFP expression (arrow). Image shows a lateral view of a stage 22 embryo, anterior to the left and dorsal to the top. (D) Embryo initially injected with Tel1-GFP mRNA and subsequently injected with Tel1 MO1 shows no GFP expression. Photo shows a lateral view of a stage 22 embryo, anterior to the left and dorsal to the top. (E) In situ hybridisation at stage 39 showing that injection of either Tel1 MO1 or Tel1 MO2 abolishes Runx1 expression in the ventral wall of the DA (red arrows). Images show lateral views with anterior to the left and dorsal to the top. Numbers of embryos represented by the image, out of the numbers analysed, are indicated in the top right corner. (F) qPCR analysis on isolated stage 35 VBIs showing no significant difference in T4- globin expression between control and Tel1-depleted embryos. (G) Embryos stained for Tie2 at stage 34 show that the development of the vasculature is affected in Tel1 morphants. Embryos exhibit a poorly developed vitelline vessel network (VitV) and an absence of aortic arches (AoArch). In contrast, although Tie2 expression levels are reduced, development of the posterior cardinal vein (PCV) and the DA are not disrupted. Embryos are shown in lateral view with anterior to the left and dorsal to the top.
Image published in: Ciau-Uitz A et al. (2010)
Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V.
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