Larger Image Fig. S3. Mapping of Prdm13 expression domain the developing neural tube. (A-C) Double in situ hybridization of Prdm13 with Eng2, Krox-20 or Mafb at the indicated stages. Krox-20 is in magenta; Mafb, in light blue. Both probes in panel A are revealed in dark blue. Note that Prdm13 is caudal to Eng2 and Krox-20, and that its expression terminates at the domain of Mafb expression. (D-H) Double labeling of Prdm13 with N-tubulin, Dbx1 or Prdm8 at the indicated stages. In panel D and F, Prdm13 is in light blue and the other probe is in dark blue. In panel E, G and H , both Prdm13 and Prdm8 are in dark blue. Note that in the neural plate Prdm13 is detected in the region of the differentiating primary interneurons, more laterally than Prdm8 and at the lateral border of the Dbx1 domain. At neural plate stage, Prdm13 is dorsal to Dbx1. Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V.
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