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Fig. 4. RFX7 and RFX4 are necessary for ciliogenesis in the neural tube. (A) Transverse section view of the neural tube at stage 23. Cilia are visualized by staining with acetylated α-tubulin (green) and ARL13B (red) antibodies. ARL13B is also expressed in cells without cilia at the neural tube. In RFX7 and RFX4 morphants, cilia were not detected. All sections from whole embryos were examined to detect cilia. White dashed lines outline the lumen of the neural tube. The number of embryos examined is indicated. (B) MOs and RNA injected embryos were collected at stage 23 and RNAs isolated from injected embryos were used to examine the gene expression. ODC was used as an internal control.

Image published in: Manojlovic Z et al. (2014)

Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V.

tuba4b.Ltuba4, alpha tubulin, alpha-tubulin, tuba4aX.laevisThroughout NF stage 23neural tube
arl13b.Ljbts8, arl2l1X.laevisThroughout NF stage 23neural tube

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