Figure 6. Ectopic Nodal cascade induction in Coco morphants targeted to the ventral-right blastomere depend on GRP Nodal. Embryos were coinjected with β-Gal lineage tracer and Coco-MO1/2 into defined regions of the dorsal or ventral right blastomere at the four-cell stage, cultured until Stage 22 (a) or 28-32 (b) and processed for Xnr1 (a) or Pitx2c (b) WMISH. (a) Very highly significant ectopic Xnr1 induction in the right LPM in all cases. (b) Very highly significant ectopic Pitx2c induction in the right LPM depended on the presence of Nodal in the targeted cells, as coinjection of Xnr1-MO into dorsal-right lateral and Coco-MO into ventral-right central regions rescued wildtype left-asymmetric Pitx2c expression. n, number of embryos analyzed.
Image published in: Tingler M et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons.
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