Fig. 6. Immunocytochemical localization of peripherin in regenerating axons. Transverse sections through the brains of frogs sacrificed 28 days (A–D) and 21 days (E,F) after optic nerve crush were immunostained with antibodies to a C-terminal peptide derived from Xenopus peripherin (diluted 1:1000: A,C,E) and to nonphosphorylated NF-M (S8 after phosphatase treatment, 1:1000: B,D; RMO270, 1:100: F). Dorsal side is up and the left side is at the left. A: At the optic chiasm, peripherin-containing fibers run longitudinally in the left, crushed optic nerve (arrow). In contrast, in the right, uncrushed optic nerve, peripherin is found only in clusters of glia cells (g). B: On a neighboring section, regenerating fibers growing outside the degenerating core (asterisk) stain strongly for NF-M. The degenerating core was not visible in A because of the level of sectioning. C: A more posterior section of the same animal as in A and B shows peripherin containing fibers in the contralateral optic tract of the diencephalon (arrow). D: A neighboring section to that in C shows the distribution of NF-M for comparison. E: In the operated optic nerve close to the chiasm, peripherin is present in the youngest regenerating axons (arrows), which are located close to the glia limitans, but is absent in the oldest regenerating axons, which are found close to the degenerating core (asterisk). F: On a section adjacent to that shown in E, NF-M is present in older regenerating axons, but is absent from young regenerating axons close to the glial limitans (dots). g, glia; oc, optic chiasm; ot, optic tract; p, pial surface; v, ventricle. Scale bars 300 m in D (applies also to A–C) and 100 m in F (applies also to E).
Image published in: Gervasi C et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| prph.L | nef4, peripherin, XIF3 | X. laevis | Throughout NF stage 66 | glial cell |
| nefm.L | nef-m, nef3, nefm-a, nefm-b, nf-m | X. laevis | Throughout NF stage 66 |
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