Figure 3 - Xenopus Foxj1 RNA misexpression in surface epithelial cells induces ectopic cilia formation. (a–f) Shown is a confocal image of the superficial epithelium in Xenopus embryos at the indicated stage, stained with antibodies to ZO-1 (red) and acetylated tubulin (green) to label cell borders and cilia, respectively. Embryos were injected at the two-cell stage with RFP RNA alone (a,c), with Foxj1 and RFP RNA (b,d), with ICD and RFP RNA (e) or with Foxj1, ICD and RFP RNA (f). Average cilia length in microns is indicated in b and d (plusminus s.d., n = 45–60 cilia from three embryos). Scale bars, 20 mum. (g,h) Transmission electron micrographs of a cilium in a multiciliated cell (g) or of an ectopic cilium (h) induced by Foxj1 RNA in an ICD background (as in f). Arrows indicate the central pair; arrowheads indicate outer dynein arms. Scale bars, 100 nm. Image redisplayed with permission from Macmillan Publishers Ltd
Image published in: Stubbs JL et al. (2008)
Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
Permanent Image Page
Printer Friendly View
XB-IMG-87029