Figure 2. In situ hybridization analysis of developmental expression of the Xenopus alpha -MHC and vMHC genes. A: Lateral view of stage 26 embryos showing alpha -MHC transcripts in the bisymmetric heart patches. B: Ventral view of embryo in A. C: Transverse section through the heart patch of stage 26 embryo. D: Lateral view of stage 30 embryo showing increased alpha -MHC expression in precardiac tissues (arrow). E: Lateral view of stage 30 embryo showing an absence of vMHC expression at this stage of development. F: Lateral view of stage 38 embryo showing alpha -MHC expression in the heart, muscles of the jaw (jm), and the lymph heart (lh). G: Transverse section through stage 36 heart shows that alpha -MHC expression is confined to the myocardium. H: Transverse section through the lymph heart (stage 38) stained for alpha -MHC. I,J: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart showing that alpha -MHC is expressed in all chambers and outflow tract. K,L: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart, showing restricted expression of vMHC in a ring around the outflow tract and the atria at the junction with the ventricle. M: Section in situ of adult Xenopus heart shows that alpha -MHC is expressed throughout the myocardium, including the atria (a) and outflow tract. N: Section in situ hybridization of adult Xenopus heart shows that vMHC is expressed in the myocardium of the outflow tract and ventricle but not the atria.
Image published in: Garriock RJ et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons.
Image source: Published
Permanent Image Page
Printer Friendly View