Larger Image

Figure 2. In situ hybridization analysis of developmental expression of the Xenopus alpha -MHC and vMHC genes. A: Lateral view of stage 26 embryos showing alpha -MHC transcripts in the bisymmetric heart patches. B: Ventral view of embryo in A. C: Transverse section through the heart patch of stage 26 embryo. D: Lateral view of stage 30 embryo showing increased alpha -MHC expression in precardiac tissues (arrow). E: Lateral view of stage 30 embryo showing an absence of vMHC expression at this stage of development. F: Lateral view of stage 38 embryo showing alpha -MHC expression in the heart, muscles of the jaw (jm), and the lymph heart (lh). G: Transverse section through stage 36 heart shows that alpha -MHC expression is confined to the myocardium. H: Transverse section through the lymph heart (stage 38) stained for alpha -MHC. I,J: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart showing that alpha -MHC is expressed in all chambers and outflow tract. K,L: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart, showing restricted expression of vMHC in a ring around the outflow tract and the atria at the junction with the ventricle. M: Section in situ of adult Xenopus heart shows that alpha -MHC is expressed throughout the myocardium, including the atria (a) and outflow tract. N: Section in situ hybridization of adult Xenopus heart shows that vMHC is expressed in the myocardium of the outflow tract and ventricle but not the atria.

Image published in: Garriock RJ et al. (2005)

Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons.

GeneSynonymsSpeciesStage(s)Tissue
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout NF stage 26heart primordium
cardiac mesoderm
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout NF stage 37 and 38right lymph heart
left lymph heart
jaw muscle
heart
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout NF stage 35 and 36myocardium
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout NF stage 29 and 30myocardium
heart
myh15.LvMHC, myh7X.laevisThroughout NF stage 45left atrium
right atrium
heart
outflow tract
myh15.LvMHC, myh7X.laevisThroughout adult frog stagecardiac ventricle
outflow tract
heart
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout NF stage 45heart
cardiac ventricle
left atrium
right atrium
cardiac mesoderm
outflow tract
myh6.Smuzak, muz, MHC-alpha, MHCalpha, ck231, aMHC, MHCa, MHC, XCMHCX.laevisThroughout adult frog stagemyocardium
left atrium
right atrium
outflow tract

Image source: Published

Permanent Image Page
Printer Friendly View

XB-IMG-1354