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Cell and Developmental Biology of Xenopus: 2019 Course at Cold Spring Harbor

2019 CSHL Cell and Developmental Biology of Xenopus/SCHOLARSHIPS AVAILABLE

April 3-16, 2018

Application Deadline: January 31, 2019

Xenopus is remarkable for modeling human diseases including birth defects, cancer, and stem cell biology. Xenopus has and continues to make a major impact in our understanding of cell and developmental biology.

Students are encouraged to target their own genes of interest using CRISPR technology and then analyze phenotypes using the diverse array of assays available in Xenopus. Specifically, techniques covered include microinjection, and various molecular manipulations including, CRISPR knockouts, morpholino based depletions, transgenics, and mRNA overexpression. In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions. To visualize subcellular and intercellular activities, we will introduce a variety of imaging methods including time-lapse, fluorescent and confocal microscopy. Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis. Biochemical approaches such as proteomics and mass spectrometry will also be discussed. This course runs in close association with two other courses: Quantitative Imaging and Protein Complexes.

This course is designed for those new to the Xenopus field, as well as for more advanced students who are interested in emerging technologies. We encourage students to bring their own genes of interest and will tailor aspects of the course to enable them to initiate studies on their specific projects. GENEROUS SCHOLARSHIPS AVAILABLE.


Lance Davidson, University of Pittsburgh
Chenbei Chang, University of Alabama at Birmingham


Mustafa Khokha, Yale University
Karen Liu, King's College London, UK

2018 Lecturers Included:

William Bement, University of Wisconsin
Ira Blitz, University of California, Irvine
Sang-Wook Cha, Cincinnati Children's Hospital Medical Center
Chenbei Chang, University of Alabama at Birmingham
Frank Conlon, University of North Carolina
Lance Davidson, University of Pittsburgh
Rebecca Heald, University of California, Berkeley
Raymond Keller, University of Virginia
Roberto Mayor, University College London, UK
Peter Nemes, University of Maryland
Gerald Thomsen, Stony Brook University
John Wallingford, University of Texas at Austin
Andrea Wills, University of Washington


Last Updated: 2019-01-09
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