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XB-IMG-2918

Xenbase Image ID: 2918

Figure 9. Immunostaining of activated Smads across the dorsoventral axis. Immunostaining of activated Smads across the dorsoventral axis. Stage 10+ embryos were fixed and bisected for immunohistochemistry. Dorsoventral orientation was determined by the dorsal blastopore lip, which is indicated with an arrow in (A-D). Embryo diameter is approximately 1 mm. Immunostaining with anti-phosphoSmad1 antibody was carried out with uninjected embryos (A) or embryos injected marginally with noggin (100 pg/embryo) (B) or BMP4 (500 pg/embryo) (C). AntiphosphoSmad2 immunostaining was performed with uninjected embryos (D) or embryos injected marginally with delta1XAR1 (1 ng/embryo) (E) or activin (50 pg/embryo) (F).

Image published in: Faure S et al. (2000)

Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
smad1.LXsmad1, smad-1, Xmad1, MLP, XMad, smad1-a, smad1-bX.laevisThroughout NF stage 10.5mesoderm
endoderm
ventral
ventral marginal zone
vegetal yolk mass
smad2.SXSmad2, smad-2X.laevisThroughout NF stage 10.5mesoderm
upper blastopore lip
endomesoderm
endoderm
involuting marginal zone
dorsal
ventral
vegetal yolk mass

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