Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2006 Feb 01;172:760-9. doi: 10.1091/mbc.e05-06-0485.
Show Gene links
Show Anatomy links
Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules.
Prunuske AJ
,
Liu J
,
Elgort S
,
Joseph J
,
Dasso M
,
Ullman KS
.
???displayArticle.abstract???
When higher eukaryotic cells transition into mitosis, the nuclear envelope, nuclear pore complexes, and nuclear lamina are coordinately disassembled. The COPI coatomer complex, which plays a major role in membrane remodeling at the Golgi, has been implicated in the process of nuclear envelope breakdown and requires interactions at the nuclear pore complex for recruitment to this new site of action at mitosis. Nup153, a resident of the nuclear pore basket, was found to be involved in COPI recruitment, but the molecular nature of the interface between COPI and the nuclear pore has not been fully elucidated. To better understand what occurs at the nuclear pore at this juncture, we have probed the role of the nucleoporin Nup358/RanBP2. Nup358 contains a repetitive zinc finger domain with overall organization similar to a region within Nup153 that is critical to COPI association, yet inspection of these two zinc finger domains reveals features that also clearly distinguish them. Here, we found that the Nup358 zinc finger domain, but not a zinc finger domain from an unrelated protein, binds to COPI and dominantly inhibits progression of nuclear envelope breakdown in an assay that robustly recapitulates this process in vitro. Moreover, the Nup358 zinc finger domain interferes with COPI recruitment to the nuclear rim. Consistent with a role for this pore protein in coordinating nuclear envelope breakdown, Nup358-specific antibodies impair nuclear disassembly. Significantly, targeting either Nup153 or Nup358 for inhibition perturbs nuclear envelope breakdown, supporting a model in which these nucleoporins play nonredundant roles, perhaps contributing to COPI recruitment platforms on both the nuclear and cytoplasmic faces of the pore. We found that an individual zinc finger is the minimal interface for COPI association, although tandem zinc fingers are optimal. These results provide new information about the critical components of nuclear membrane remodeling and lay the foundation for a better understanding of how this process is regulated.
Alam,
Ubiquitin interactions of NZF zinc fingers.
2004, Pubmed
Alam,
Ubiquitin interactions of NZF zinc fingers.
2004,
Pubmed
Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase
Ball,
Versatility at the nuclear pore complex: lessons learned from the nucleoporin Nup153.
2005,
Pubmed
Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed
Bernad,
Nup358/RanBP2 attaches to the nuclear pore complex via association with Nup88 and Nup214/CAN and plays a supporting role in CRM1-mediated nuclear protein export.
2004,
Pubmed
,
Xenbase
Buendia,
Domain-specific disassembly and reassembly of nuclear membranes during mitosis.
1997,
Pubmed
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Dimaano,
RNA association defines a functionally conserved domain in the nuclear pore protein Nup153.
2001,
Pubmed
,
Xenbase
Drummond,
Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.
1999,
Pubmed
,
Xenbase
Ellenberg,
Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis.
1997,
Pubmed
Fahrenkrog,
Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex.
2002,
Pubmed
,
Xenbase
Fahrenkrog,
The nuclear pore complex: a jack of all trades?
2004,
Pubmed
Forler,
RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export.
2004,
Pubmed
Gruenbaum,
The nuclear lamina comes of age.
2005,
Pubmed
Joseph,
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
2004,
Pubmed
Joseph,
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
2002,
Pubmed
Liu,
The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153.
2003,
Pubmed
,
Xenbase
Lohka,
Analysis of nuclear envelope assembly using extracts of Xenopus eggs.
1998,
Pubmed
,
Xenbase
Macaulay,
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
1996,
Pubmed
,
Xenbase
Margalit,
Breaking and making of the nuclear envelope.
2005,
Pubmed
Panté,
Nuclear pore complex structure: unplugged and dynamic pores.
2004,
Pubmed
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Powers,
Preparation and use of interphase Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Prunuske,
The nuclear envelope: form and reformation.
2006,
Pubmed
Rosenblatt,
Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly.
2004,
Pubmed
Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed
Saitoh,
Direct and indirect association of the small GTPase ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts.
1996,
Pubmed
,
Xenbase
Salina,
Nup358 integrates nuclear envelope breakdown with kinetochore assembly.
2003,
Pubmed
Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed
Suntharalingam,
Peering through the pore: nuclear pore complex structure, assembly, and function.
2003,
Pubmed
Vigers,
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs.
1991,
Pubmed
,
Xenbase
Walther,
The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.
2002,
Pubmed
,
Xenbase
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Wang,
Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4.
2003,
Pubmed
Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
Yang,
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
1998,
Pubmed
,
Xenbase
Yang,
Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.
1997,
Pubmed
