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Mol Biol Rep
2009 Mar 01;363:501-11. doi: 10.1007/s11033-007-9207-y.
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Molecular cloning and characterization of follistatin in the gilthead sea bream, Sparus aurata.
Funkenstein B
,
Rebhan Y
,
Skopal T
.
???displayArticle.abstract??? Follistatin (FST) is an activin-binding protein that neutralizes the activity of activin. FST also binds other members of the transforming growth factor-beta (TGF-beta) superfamily, including myostatin (MSTN). We report herein on the isolation and characterization of a full-length cDNA sequence predicted to encode FST in a marine fish, the gilthead sea bream Sparus aurata. The deduced amino acid sequence of sea bream FST (saFST) is highly conserved to the counterpart sequences in other vertebrates and contains the N-terminal domain and three FST domains. The deduced mature saFST shows 81-86% identity with FSTs from other vertebrates. It is 290 amino acids long, similar to other fish FSTs and the short isoform of Xenopus FST but longer by two residues than mammalian FST288. Ontogeny of MSTN (a TGF-beta superfamily member and a negative growth regulator of skeletal muscle in mammals), and FST (known to bind MSTN) gene expression revealed the presence of both transcripts throughout larval development. However, a different expression pattern was found in earlier developmental stages; while MSTN could not be detected prior to the day of hatching, FST transcript was detected in embryos 12 h post-fertilization, confirming its role during vertebrate embryonic development. Both FST and MSTN were expressed in many adult tissues, with variable levels of expression, including muscle. Recombinant saFST inhibited saMSTN activity in a reporter gene assay, indicating a similar effect to that reported in mammals.
Acosta,
Myostatin gene silenced by RNAi show a zebrafish giant phenotype.
2005, Pubmed
Acosta,
Myostatin gene silenced by RNAi show a zebrafish giant phenotype.
2005,
Pubmed
Albano,
Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain.
1994,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Amthor,
The regulation and action of myostatin as a negative regulator of muscle development during avian embryogenesis.
2002,
Pubmed
Amthor,
A molecular mechanism enabling continuous embryonic muscle growth - a balance between proliferation and differentiation.
1999,
Pubmed
Amthor,
Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis.
2004,
Pubmed
Amthor,
Follistatin regulates bone morphogenetic protein-7 (BMP-7) activity to stimulate embryonic muscle growth.
2002,
Pubmed
Amthor,
The expression and regulation of follistatin and a follistatin-like gene during avian somite compartmentalization and myogenesis.
1996,
Pubmed
Armand,
Expression and neural control of follistatin versus myostatin genes during regeneration of mouse soleus.
2003,
Pubmed
Bauer,
Follistatin and noggin are excluded from the zebrafish organizer.
1998,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Davis,
Expression pattern of messenger ribonucleic acid for follistatin and the inhibin/activin subunits during follicular and testicular development in gallus domesticus.
1998,
Pubmed
Fainsod,
The dorsalizing and neural inducing gene follistatin is an antagonist of BMP-4.
1997,
Pubmed
,
Xenbase
Funkenstein,
Molecular cloning of fish alcohol dehydrogenase cDNA.
1996,
Pubmed
Funkenstein,
Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata).
2007,
Pubmed
Funkenstein,
Cloning of putative piscine (Sparus aurata) transthyretin: developmental expression and tissue distribution.
1999,
Pubmed
Funkenstein,
Expression, purification, renaturation and activation of fish myostatin expressed in Escherichia coli: facilitation of refolding and activity inhibition by myostatin prodomain.
2007,
Pubmed
Gamer,
A novel BMP expressed in developing mouse limb, spinal cord, and tail bud is a potent mesoderm inducer in Xenopus embryos.
1999,
Pubmed
,
Xenbase
Graham,
Interactions between rhombomeres modulate Krox-20 and follistatin expression in the chick embryo hindbrain.
1996,
Pubmed
,
Xenbase
Gregory,
Cloning and characterization of myogenic regulatory genes in three Ictalurid species.
2004,
Pubmed
Grobet,
A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle.
1997,
Pubmed
Grobet,
Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle.
1998,
Pubmed
Helterline,
Embryonic and tissue-specific regulation of myostatin-1 and -2 gene expression in zebrafish.
2007,
Pubmed
Hemmati-Brivanlou,
Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity.
1994,
Pubmed
,
Xenbase
Hill,
The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.
2002,
Pubmed
Hill,
Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains.
2003,
Pubmed
Iemura,
Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo.
1998,
Pubmed
,
Xenbase
Kambadur,
Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle.
1997,
Pubmed
Keutmann,
The role of follistatin domains in follistatin biological action.
2004,
Pubmed
Kocabas,
Molecular characterization and differential expression of the myostatin gene in channel catfish (Ictalurus punctatus).
2002,
Pubmed
Kozak,
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
1981,
Pubmed
Lee,
Regulation of myostatin activity and muscle growth.
2001,
Pubmed
Lin,
Regulation of ovarian function by the TGF-beta superfamily and follistatin.
2003,
Pubmed
Maccatrozzo,
A novel second myostatin gene is present in teleost fish.
2001,
Pubmed
Maccatrozzo,
Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern.
2001,
Pubmed
Matzuk,
Multiple defects and perinatal death in mice deficient in follistatin.
1995,
Pubmed
,
Xenbase
McPherron,
Double muscling in cattle due to mutations in the myostatin gene.
1997,
Pubmed
McPherron,
Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.
1997,
Pubmed
Michel,
Rat follistatin: ontogeny of steady-state mRNA levels in different tissues predicts organ-specific functions.
1991,
Pubmed
Michel,
Follistatins: more than follicle-stimulating hormone suppressing proteins.
1993,
Pubmed
Nakamura,
Activin-binding protein from rat ovary is follistatin.
1990,
Pubmed
Østbye,
The two myostatin genes of Atlantic salmon (Salmo salar) are expressed in a variety of tissues.
2001,
Pubmed
Radaelli,
Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study.
2003,
Pubmed
Rescan,
Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss).
2001,
Pubmed
Roberts,
Differential skeletal muscle expression of myostatin across teleost species, and the isolation of multiple myostatin isoforms.
2001,
Pubmed
Rodgers,
Isolation and characterization of myostatin complementary deoxyribonucleic acid clones from two commercially important fish: Oreochromis mossambicus and Morone chrysops.
2001,
Pubmed
Saito,
Differential biosynthesis and intracellular transport of follistatin isoforms and follistatin-like-3.
2005,
Pubmed
Shimasaki,
Primary structure of the human follistatin precursor and its genomic organization.
1988,
Pubmed
Sidis,
Follistatin: essential role for the N-terminal domain in activin binding and neutralization.
2001,
Pubmed
Tashiro,
Expression of mRNA for activin-binding protein (follistatin) during early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Thies,
GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor binding.
2001,
Pubmed
,
Xenbase
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tuuri,
The tissue distribution of activin beta A- and beta B-subunit and follistatin messenger ribonucleic acids suggests multiple sites of action for the activin-follistatin system during human development.
1994,
Pubmed
Welt,
Activins, inhibins, and follistatins: from endocrinology to signaling. A paradigm for the new millennium.
2002,
Pubmed
Xu,
Analysis of myostatin gene structure, expression and function in zebrafish.
2003,
Pubmed
Zimmers,
Induction of cachexia in mice by systemically administered myostatin.
2002,
Pubmed
