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XB-ART-58869
J Cell Biol 2022 Jul 04;2217:. doi: 10.1083/jcb.202008116.
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Cell cortex regulation by the planar cell polarity protein Prickle1.

Huang Y , Winklbauer R .


Abstract
The planar cell polarity pathway regulates cell polarity, adhesion, and rearrangement. Its cytoplasmic core components Prickle (Pk) and Dishevelled (Dvl) often localize as dense puncta at cell membranes to form antagonizing complexes and establish cell asymmetry. In vertebrates, Pk and Dvl have been implicated in actomyosin cortex regulation, but the mechanism of how these proteins control cell mechanics is unclear. Here we demonstrate that in Xenopus prechordal mesoderm cells, diffusely distributed, cytoplasmic Pk1 up-regulates the F-actin content of the cortex. This counteracts cortex down-regulation by Dvl2. Both factors act upstream of casein kinase II to increase or decrease cortical tension. Thus, cortex modulation by Pk1 and Dvl2 is translated into mechanical force and affects cell migration and rearrangement during radial intercalation in the prechordal mesoderm. Pk1 also forms puncta and plaques, which are associated with localized depletion of cortical F-actin, suggesting opposite roles for diffuse and punctate Pk1.

PubMed ID: 35512799
PMC ID: PMC9082893
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dvl2 prickle1 prok1
GO keywords: cytoskeleton [+]
Morpholinos: dvl2 MO1 prickle1 MO1

References [+] :
Adler, Planar signaling and morphogenesis in Drosophila. 2002, Pubmed