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Methods Mol Biol 2022 Jan 01;2438:147-161. doi: 10.1007/978-1-0716-2035-9_10.
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Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm.

Ossipova O , Mancini P , Sokol SY .

Planar cell polarity (PCP) refers to coordinated cell polarization in the plane of the tissue. Genetic studies in Drosophila identified several core PCP genes, whose products function together in a signaling pathway that regulates cell shape, epithelial tissue organization and remodeling during morphogenesis. PCP is detected by the asymmetric distribution of core PCP proteins at different borders of epithelial cells. Believed to be critical for signaling, this segregation is studied by a variety of techniques, such as direct immunostaining and imaging of fluorescent PCP protein fusions or fluorescence recovery after photobleaching (FRAP). All of the above techniques can be applied to the analysis of the Xenopus neural plate to study the dynamics of tissue polarization, making this system one of the best vertebrate PCP models. This chapter describes how to image PCP proteins in Xenopus neuroectoderm for both fixed and live samples. These robust cellular techniques will contribute to mechanistic studies of PCP in vertebrate embryos.

PubMed ID: 35147941
Article link: Methods Mol Biol
Grant support: [+]

Species referenced: Xenopus laevis

References [+] :
Butler, Planar cell polarity in development and disease. 2017, Pubmed