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Fig. 1. Identification of a Xath5 regulatory fragment that functions in vivo. (A) The pG1X5 construct contains 3.3 kb of 5′ Xath5a genomic DNA cloned into the promoterless vector pG1 with a GFP reporter. The TATAA element is at –226 bp, the putative transcription start site is at– 205 bp and the translation start site is at +1 bp. (B) In situ hybridization showing that endogenous Xath5 mRNA is expressed in the retina, the pineal gland (arrowhead), and the olfactory placodes (arrows) of a stage 28 embryo (frontal view). (C,D) Frontal and lateral views of a stage 28 transgenic pG1X5 embryo showing expression of the transgene in the same tissues as the endogenous Xath5 mRNA (compare with B).

Image published in: Hutcheson DA et al. (2005)

Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
atoh7.Sath5, atoh7-a, atoh7-b, math5, Xath5, Xath5bX. laevisThroughout NF stage 28retina
pineal gland
olfactory placode
eye

Image source: Published

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