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Genesis
2011 Mar 01;493:117-23. doi: 10.1002/dvg.20689.
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Use of fully modified 2'-O-methyl antisense oligos for loss-of-function studies in vertebrate embryos.
Schneider PN
,
Olthoff JT
,
Matthews AJ
,
Houston DW
.
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Antisense oligonucleotides are commonly employed to study the roles of genes in development. Although morpholino phosphorodiamidate oligonucleotides (morpholinos) are widely used to block translation or splicing of target gene products' the usefulness of other modifications in mediating RNase-H independent inhibition of gene activity in embryos has not been investigated. In this study, we investigated the extent that fully modified 2'-O-methyl oligonucleotides (2'-OMe oligos) that can function as translation inhibiting reagents in vivo, using Xenopus and zebrafish embryos. We find that oligos against Xenopus β-catenin, wnt11, and bmp4 and against zebrafish chordin (chd), which can efficiently and specifically generate embryonic loss-of-function phenotypes comparable with morpholino injection and other methods. These results show that fully modified 2'-OMe oligos can function as RNase-H independent antisense reagents in vertebrate embryos and can thus serve as an alternative modification to morpholinos in some cases.
Boiziau,
Inhibition of translation initiation by antisense oligonucleotides via an RNase-H independent mechanism.
1991, Pubmed,
Xenbase
Boiziau,
Inhibition of translation initiation by antisense oligonucleotides via an RNase-H independent mechanism.
1991,
Pubmed
,
Xenbase
Cazenave,
Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.
1987,
Pubmed
,
Xenbase
Cuykendall,
Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formation.
2009,
Pubmed
,
Xenbase
Dash,
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
1987,
Pubmed
,
Xenbase
Eisen,
Controlling morpholino experiments: don't stop making antisense.
2008,
Pubmed
,
Xenbase
Gawantka,
Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1.
1995,
Pubmed
,
Xenbase
Hammerschmidt,
dino and mercedes, two genes regulating dorsal development in the zebrafish embryo.
1996,
Pubmed
Heasman,
Morpholino oligos: making sense of antisense?
2002,
Pubmed
,
Xenbase
Heasman,
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase
Horwich,
The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC.
2007,
Pubmed
Hukriede,
Conserved requirement of Lim1 function for cell movements during gastrulation.
2003,
Pubmed
,
Xenbase
Hulstrand,
The use of antisense oligonucleotides in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Hutvágner,
Sequence-specific inhibition of small RNA function.
2004,
Pubmed
Kerr,
Maternal Tgif1 regulates nodal gene expression in Xenopus.
2008,
Pubmed
,
Xenbase
Kimmel,
Stages of embryonic development of the zebrafish.
1995,
Pubmed
,
Xenbase
Lennox,
Characterization of modified antisense oligonucleotides in Xenopus laevis embryos.
2006,
Pubmed
,
Xenbase
Maden,
The numerous modified nucleotides in eukaryotic ribosomal RNA.
1990,
Pubmed
Manoharan,
2'-carbohydrate modifications in antisense oligonucleotide therapy: importance of conformation, configuration and conjugation.
1999,
Pubmed
Nasevicius,
Effective targeted gene 'knockdown' in zebrafish.
2000,
Pubmed
Pandur,
Wnt-11 activation of a non-canonical Wnt signalling pathway is required for cardiogenesis.
2002,
Pubmed
,
Xenbase
Reversade,
Depletion of Bmp2, Bmp4, Bmp7 and Spemann organizer signals induces massive brain formation in Xenopus embryos.
2005,
Pubmed
,
Xenbase
Rupp,
Ubiquitous MyoD transcription at the midblastula transition precedes induction-dependent MyoD expression in presumptive mesoderm of X. laevis.
1991,
Pubmed
,
Xenbase
Sazani,
Therapeutic potential of antisense oligonucleotides as modulators of alternative splicing.
2003,
Pubmed
Shuttleworth,
Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.
1988,
Pubmed
,
Xenbase
Stein,
A specificity comparison of four antisense types: morpholino, 2'-O-methyl RNA, DNA, and phosphorothioate DNA.
1997,
Pubmed
Tételin,
Xenopus Wnt11b is identified as a potential pronephric inducer.
2010,
Pubmed
,
Xenbase
Yang,
HEN1 recognizes 21-24 nt small RNA duplexes and deposits a methyl group onto the 2' OH of the 3' terminal nucleotide.
2006,
Pubmed