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PLoS One January 1, 2012; 7 (5): e36855.
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Ventx factors function as Nanog-like guardians of developmental potential in Xenopus.

Scerbo P , Girardot F , Vivien C , Markov GV , Luxardi G , Demeneix B , Kodjabachian L , Coen L .

Vertebrate development requires progressive commitment of embryonic cells into specific lineages through a continuum of signals that play off differentiation versus multipotency. In mammals, Nanog is a key transcription factor that maintains cellular pluripotency by controlling competence to respond to differentiation cues. Nanog orthologs are known in most vertebrates examined to date, but absent from the Anuran amphibian Xenopus. Interestingly, in silico analyses and literature scanning reveal that basal vertebrate ventral homeobox (ventxs) and mammalian Nanog factors share extensive structural, evolutionary and functional properties. Here, we reassess the role of ventx activity in Xenopus laevis embryos and demonstrate that they play an unanticipated role as guardians of high developmental potential during early development. Joint over-expression of Xenopus ventx1.2 and ventx2.1-b (ventx1/2) counteracts lineage commitment towards both dorsal and ventral fates and prevents msx1-induced ventralization. Furthermore, ventx1/2 inactivation leads to down-regulation of the multipotency marker oct91 and to premature differentiation of blastula cells. Finally, supporting the key role of ventx1/2 in the control of developmental potential during development, mouse Nanog (mNanog) expression specifically rescues embryonic axis formation in ventx1/2 deficient embryos. We conclude that during Xenopus development ventx1/2 activity, reminiscent of that of Nanog in mammalian embryos, controls the switch of early embryonic cells from uncommitted to committed states.

PubMed ID: 22606298
PMC ID: PMC3351468
Article link: PLoS One

Species referenced: Xenopus laevis
Genes referenced: egr2 gsc hba4 hhex hoxb9 mixer msx1 myf5 nf2 nodal5.2 nodal5.4 pou5f3.1 shh sia1 six6 tfap2a ventx1.1 ventx1.2 ventx2.1 ventx2.2 wnt8a

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References [+] :
Abu-Remaileh, Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling. 2010, Pubmed, Xenbase