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J Cell Biol March 16, 2015; 208 (6): 839-56.

PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.

Luu O , Damm EW , Parent SE , Barua D , Smith TH , Wen JW , Lepage SE , Nagel M , Ibrahim-Gawel H , Huang Y , Bruce AE , Winklbauer R .

Cleft-like boundaries represent a type of cell sorting boundary characterized by the presence of a physical gap between tissues. We studied the cleft-like ectoderm-mesoderm boundary in Xenopus laevis and zebrafish gastrulae. We identified the transcription factor Snail1 as being essential for tissue separation, showed that its expression in the mesoderm depends on noncanonical Wnt signaling, and demonstrated that it enables paraxial protocadherin (PAPC) to promote tissue separation through two novel functions. First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation. Second, PAPC controls formation of a distinct type of adhesive contact between mesoderm and ectoderm cells that shows properties of a cleft-like boundary at the single-cell level. It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps. These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

PubMed ID: 25778923
PMC ID: PMC4362454
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: bcr cdh3 daam1 dvl2 efnb1 ephb4 fzd7 jun mapk8 pcdh8 pcdh8.2 pklr prickle1 rhoa rnf121 snai1 vangl2
Morpholinos: daam1 MO1 dvl2 MO1 ephb4 MO1 fzd7 MO1 fzd7 MO4 fzd7 MO5 jun MO1 jun MO2 pcdh8.2 MO2 pcdh8.2 MO3 prickle1 MO1 snai1 MO3 vangl2 MO2

Article Images: [+] show captions
References [+] :
Barrallo-Gimeno, The Snail genes as inducers of cell movement and survival: implications in development and cancer. 2005, Pubmed