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XB-ART-51156
Development October 1, 2015; 142 (19): 3429-39.
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Control of vertebrate core planar cell polarity protein localization and dynamics by Prickle 2.

Butler MT , Wallingford JB .


Abstract
Planar cell polarity (PCP) is a ubiquitous property of animal tissues and is essential for morphogenesis and homeostasis. In most cases, this fundamental property is governed by a deeply conserved set of ''core PCP'' proteins, which includes the transmembrane proteins Van Gogh-like (Vangl) and Frizzled (Fzd), as well as the cytoplasmic effectors Prickle (Pk) and Dishevelled (Dvl). Asymmetric localization of these proteins is thought to be central to their function, and understanding the dynamics of these proteins is an important challenge in developmental biology. Among the processes that are organized by the core PCP proteins is the directional beating of cilia, such as those in the vertebrate node, airway and brain. Here, we exploit the live imaging capabilities of Xenopus to chart the progressive asymmetric localization of fluorescent reporters of Dvl1, Pk2 and Vangl1 in a planar polarized ciliated epithelium. Using this system, we also characterize the influence of Pk2 on the asymmetric dynamics of Vangl1 at the cell cortex, and we define regions of Pk2 that control its own localization and those impacting Vangl1. Finally, our data reveal a striking uncoupling of Vangl1 and Dvl1 asymmetry. This study advances our understanding of conserved PCP protein functions and also establishes a rapid, tractable platform to facilitate future in vivo studies of vertebrate PCP protein dynamics.

PubMed ID: 26293301
PMC ID: PMC4631750
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: celsr2 celsr3 dvl1 dvl2 dvl3 fzd6 fzd7 fzd8 invs prickle1 prickle2 prok2 vangl1 vangl2
Morpholinos: prickle2 MO1 prickle2 MO2

References [+] :
Adler, The domineering non-autonomy of frizzled and van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling. 2000, Pubmed