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XB-ART-52451
Elife September 13, 2016; 5

Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis.

Walentek P , Quigley IK , Sun DI , Sajjan UK , Kintner C , Harland RM .


Abstract
Upon cell cycle exit, centriole-to-basal body transition facilitates cilia formation. The centriolar protein Cp110 is a regulator of this process and cilia inhibitor, but its positive roles in ciliogenesis remain poorly understood. Using Xenopus we show that Cp110 inhibits cilia formation at high levels, while optimal levels promote ciliogenesis. Cp110 localizes to cilia-forming basal bodies and rootlets, and is required for ciliary adhesion complexes that facilitate Actin interactions. The opposing roles of Cp110 in ciliation are generated in part by coiled-coil domains that mediate preferential binding to centrioles over rootlets. Because of its dual role in ciliogenesis, Cp110 levels must be precisely controlled. In multiciliated cells, this is achieved by both transcriptional and post-transcriptional regulation through ciliary transcription factors and microRNAs, which activate and repress cp110 to produce optimal Cp110 levels during ciliogenesis. Our data provide novel insights into how Cp110 and its regulation contribute to development and cell function.

PubMed ID: 27623009
PMC ID: PMC5045295
Article link: Elife
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ccp110 cdc20b cep97 cfap20 cfp foxj1.2 gnl3 herpud1 mcc mcidas nkx2-2 pax6 pitx2 pxn tub


Article Images: [+] show captions
References [+] :
Antoniades, Making the connection: ciliary adhesion complexes anchor basal bodies to the actin cytoskeleton. 2014, Pubmed