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XB-ART-53984
Development January 1, 2017; 144 (20): 3755-3765.

Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex.

Aguero T , Jin Z , Chorghade S , Kalsotra A , King ML , Yang J .


Abstract
In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.

PubMed ID: 28870987
PMC ID: PMC5675448
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: dazl ddx25 ddx4 ddx59 dnd1 eif3a eif3c eif3f eif3m gdf1 germes grip2 myc nanos1 odc1 pgat pgc piwil1 pum2 sybu trim36 tubb3 vegt wnt11b
GO keywords: germ cell development [+]
Antibodies: Ddx4 Ab3 Dnd1 Ab1 Eif3c Ab1 Nanos1 Ab1 Pum2 Ab1 Tubb3 Ab2 eif3f Ab1 piwil1 Ab1


Article Images: [+] show captions
References [+] :
Ahringer, Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3' untranslated region. 1991, Pubmed