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XB-ART-54309
Development January 1, 2018; 145 (1):

A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome.

Butler AM , Owens DA , Wang L , King ML .


Abstract
Xenopus primordial germ cells (PGCs) are determined by the presence of maternally derived germ plasm. Germ plasm components both protect PGCs from somatic differentiation and begin a unique gene expression program. Segregation of the germline from the endodermal lineage occurs during gastrulation, and PGCs subsequently initiate zygotic transcription. However, the gene network(s) that operate to both preserve and promote germline differentiation are poorly understood. Here, we utilized RNA-sequencing analysis to comprehensively interrogate PGC and neighboring endoderm cell mRNAs after lineage segregation. We identified 1865 transcripts enriched in PGCs compared with endoderm cells. We next compared the PGC-enriched transcripts with previously identified maternal, vegetally enriched transcripts and found that ∼38% of maternal transcripts were enriched in PGCs, including sox7 PGC-directed sox7 knockdown and overexpression studies revealed an early requirement for sox7 in germ plasm localization, zygotic transcription and PGC number. We identified pou5f3.3 as the most highly expressed and enriched POU5F1 homolog in PGCs. We compared the Xenopus PGC transcriptome with human PGC transcripts and showed that 80% of genes are conserved, underscoring the potential usefulness of Xenopus for understanding human germline specification.

PubMed ID: 29158442
PMC ID: PMC5825864
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: bpnt2 cdc20 ctrl dazl ddx4 dnd1 fam168b fer fes germes grip2 h1-8 lmnb3 nasp odc1 pcna pgam1 pgat pgc piwil1 pou5f3.3 pphln1 ppp1r2 prpsap2 rtn3 sox7 tspan1 zfyve26
GO keywords: germ cell development
Antibodies: piwil1 Ab1
Morpholinos: pou5f3.3 MO7 sox7 MO2

GEO Series: GSE102047: Xenbase,  NCBI

Article Images: [+] show captions
References [+] :
Aguero, Mechanisms of Vertebrate Germ Cell Determination. 2017, Pubmed, Xenbase