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Genesis January 1, 2018; 56 (6-7): e23108.
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Regulation of neural crest development by the formin family protein Daam1.

Ossipova O , Kerney R , Saint-Jeannet JP , Sokol SY .

The neural crest (NC) multipotent progenitor cells form at the neural plate border and migrate to diverse locations in the embryo to differentiate into many cell types. NC is specified by several embryonic pathways, however the role of noncanonical Wnt signaling in this process remains poorly defined. Daam1 is a formin family protein that is present in embryonic ectoderm at the time of NC formation and can mediate noncanonical Wnt signaling. Our interference experiments indicated that Daam1 is required for NC gene activation. To further study the function of Daam1 in NC development we used a transgenic reporter Xenopus line, in which GFP transcription is driven by sox10 upstream regulatory sequences. The activation of the sox10:GFP reporter in a subset of NC cells was suppressed after Daam1 depletion and in embryos expressing N-Daam1, a dominant interfering construct. Moreover, N-Daam1 blocked reporter activation in neuralized ectodermal explants in response to Wnt11, but not Wnt8 or Wnt3a, confirming that the downstream pathways are different. In complementary experiments, a constitutively active Daam1 fragment expanded the NC territory, but this gain-of-function activity was eliminated in a construct with a point mutation in the FH2 domain that is critical for actin polymerization. These observations suggest a new role of Daam1 and actin remodeling in NC specification.

PubMed ID: 29673042
PMC ID: PMC6105563
Article link: Genesis
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: chrd.1 daam1 fmn1 foxd3 h2bc21 myod1 nrp1 pax3 snai2 sox10 sox2 sox8 twist1 wnt11 wnt11b wnt3a wnt8a
GO keywords: actin polymerization or depolymerization [+]

Article Images: [+] show captions
References [+] :
Ang, DAAM1 is a formin required for centrosome re-orientation during cell migration. 2011, Pubmed, Xenbase