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XB-ART-55075
Mol Cell Proteomics January 1, 2018; 17 (10): 1991-2004.
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Proteomic Profiling of Microtubule Self-organization in M-phase.

Rosas-Salvans M , Cavazza T , Espadas G , Sabido E , Vernos I .


Abstract
Microtubules (MTs) and associated proteins can self-organize into complex structures such as the bipolar spindle, a process in which RanGTP plays a major role. Addition of RanGTP to M-phase Xenopus egg extracts promotes the nucleation and self-organization of MTs into asters and bipolar-like structures in the absence of centrosomes or chromosomes. We show here that the complex proteome of these RanGTP-induced MT assemblies is similar to that of mitotic spindles. Using proteomic profiling we show that MT self-organization in the M-phase cytoplasm involves the non-linear and non-stoichiometric recruitment of proteins from specific functional groups. Our study provides for the first time a temporal understanding of the protein dynamics driving MT self-organization in M-phase.

PubMed ID: 29970457
PMC ID: PMC6166669
Article link: Mol Cell Proteomics



References [+] :
Bonner, Mitotic spindle proteomics in Chinese hamster ovary cells. 2011, Pubmed