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Int J Mol Sci April 16, 2019; 20 (8):

Calcium Activity Dynamics Correlate with Neuronal Phenotype at a Single Cell Level and in a Threshold-Dependent Manner.

Paudel S , Ablondi E , Sehdev M , Marken J , Halleran A , Rahman A , Kemper P , Saha MS .

Calcium is a ubiquitous signaling molecule that plays a vital role in many physiological processes. Recent work has shown that calcium activity is especially critical in vertebrate neural development. Here, we investigated if calcium activity and neuronal phenotype are correlated only on a population level or on the level of single cells. Using Xenopus primary cell culture in which individual cells can be unambiguously identified and associated with a molecular phenotype, we correlated calcium activity with neuronal phenotype on the single-cell level. This analysis revealed that, at the neural plate stage, a high frequency of low-amplitude spiking activity correlates with an excitatory, glutamatergic phenotype, while high-amplitude spiking activity correlates with an inhibitory, GABAergic phenotype. Surprisingly, we also found that high-frequency, low-amplitude spiking activity correlates with neural progenitor cells and that differentiating cells exhibit higher spike amplitude. Additional methods of analysis suggested that differentiating marker tubb2b-expressing cells exhibit relatively persistent and predictable calcium activity compared to the irregular activity of neural progenitor cells. Our study highlights the value of using a range of thresholds for analyzing calcium activity data and underscores the importance of employing multiple methods to characterize the often irregular, complex patterns of calcium activity during early neural development.

PubMed ID: 30995769
PMC ID: PMC6515432
Article link: Int J Mol Sci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: gad1.1 slc17a7 sox2 tubb2b
GO keywords: nervous system development

Article Images: [+] show captions
References [+] :
Abdul-Wajid, T-type Calcium Channel Regulation of Neural Tube Closure and EphrinA/EPHA Expression. 2016, Pubmed, Xenbase