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XB-ART-56302
Nat Commun January 1, 2019; 10 (1): 4269.

Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals.

Gentsch GE , Spruce T , Owens NDL , Smith JC .


Abstract
Embryonic development yields many different cell types in response to just a few families of inductive signals. The property of signal-receiving cells that determines how they respond to inductive signals is known as competence, and it differs in different cell types. Here, we explore the ways in which maternal factors modify chromatin to specify initial competence in the frog Xenopus tropicalis. We identify early-engaged regulatory DNA sequences, and infer from them critical activators of the zygotic genome. Of these, we show that the pioneering activity of the maternal pluripotency factors Pou5f3 and Sox3 determines competence for germ layer formation by extensively remodelling compacted chromatin before the onset of inductive signalling. This remodelling includes the opening and marking of thousands of regulatory elements, extensive chromatin looping, and the co-recruitment of signal-mediating transcription factors. Our work identifies significant developmental principles that inform our understanding of how pluripotent stem cells interpret inductive signals.

PubMed ID: 31537794
PMC ID: PMC6753111
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: actc1 cdc25b ctnnb1 eomes foxh1.2 myod1 nodal pkd2 pou5f3.1 pou5f3.3 smad1 smad2 sox2 sox3 tbxt
GO keywords: formation of primary germ layer
Antibodies: Tbx6 Ab1

GEO Series: GSE113186: Xenbase,  NCBI

Article Images: [+] show captions
References [+] :
Balwierz, ISMARA: automated modeling of genomic signals as a democracy of regulatory motifs. 2014, Pubmed