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J Biol Chem January 1, 2020; 295 (26): 8759-8774.
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eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled.

Wesslowski J , Kozielewicz P , Wang X , Cui H , Schihada H , Kranz D , Karuna M P , Levkin P , Gross JC , Boutros M , Schulte G , Davidson G .

)/Wntless-dependent manner, activated Wnt/β-catenin signaling in 2D and 3D cell culture experiments, promoted axis duplication in Xenopus embryos, stimulated low-density lipoprotein receptor-related protein 6 (LRP6) phosphorylation in cells, and associated with exosomes. Further, we used conditioned medium containing eGFP-Wnt-3a to visualize its binding to FZD and to quantify Wnt-FZD interactions in real time in live cells, utilizing a recently established NanoBRET-based ligand binding assay. In summary, the development of a biologically active, fluorescent Wnt-3a reported here opens up the technical possibilities to unravel the intricate biology of Wnt signaling and Wnt-receptor selectivity.

PubMed ID: 32381507
PMC ID: PMC7324525
Article link: J Biol Chem

Species referenced: Xenopus
Genes referenced: ctnnb1 fzd2 fzd4 fzd8 lrp6 pdcd6ip sdcbp wls wnt3a
GO keywords: frizzled binding [+]

Article Images: [+] show captions
References [+] :
Arthofer, WNT Stimulation Dissociates a Frizzled 4 Inactive-State Complex with Gα12/13. 2017, Pubmed