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XB-ART-28614
Mol Cell Biol 1986 Jul 01;67:2543-50.
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Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.

Bogenhagen DF , Yoza BK .


Abstract
The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites previously mapped as initiation sites for transcription in vivo. At each of the four sites, initiation occurred within a conserved nucleotide sequence, ACPuTTATA. This consensus sequence is not related to promoters for transcription of human mtDNA.

PubMed ID: 3023938
PMC ID: PMC367809
Article link: Mol Cell Biol



References [+] :
Anderson, Sequence and organization of the human mitochondrial genome. 1981, Pubmed