Gonsalvez GB , Long RM .

	Figure 1. A general pathway for cytoplasmic RNA localization can be broken into distinct stepsStep 1 – RNA localization substrates (green line) can be identified in the nucleus by RNA-binding proteins (purple ball). Step 2 – the RNA-protein complex is exported from the nucleus to the cytoplasm through the nuclear pore complex. Step 3 – once in the cytoplasm, the RNA can associate with additional proteins (red) that interface the RNA with motor proteins (blue) that transport the RNA along cytoskeletal filaments (gray) to the site of localization. Step 4 – upon reaching the correct destination, translational repression is relieved and the corresponding protein (green ball) is synthesized at the site of action.
	Figure 2. ASH1 mRNA localization in S. cerevisiaeThe distribution of ASH1 mRNA is shown in red, and the positions of the nuclei are shown in blue.
	Figure 3. mRNA localization in Drosophila oocytesA schematic representation of the Drosophila egg chamber (top panel) oskar mRNA is transcribed in the nurse cells, transported into the oocyte and localized at the posterior pole (red). The bottom panel shows oskar mRNA (red) localization as detected by in situ hybridization. DAPI stained DNA is shown in blue.

Andoh, Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip. 2006, Pubmed

Andoh, Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip. 2006, Pubmed
Aronov, mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae. 2007, Pubmed
Barbee, Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies. 2006, Pubmed
Beach, Localization and anchoring of mRNA in budding yeast. 1999, Pubmed
Berleth, The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo. 1988, Pubmed
Bertrand, Localization of ASH1 mRNA particles in living yeast. 1998, Pubmed
Besse, Drosophila PTB promotes formation of high-order RNP particles and represses oskar translation. 2009, Pubmed
Betley, A ubiquitous and conserved signal for RNA localization in chordates. 2002, Pubmed , Xenbase
Betley, Kinesin II mediates Vg1 mRNA transport in Xenopus oocytes. 2004, Pubmed , Xenbase
Bobola, Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells. 1996, Pubmed
Böhl, She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p. 2000, Pubmed
Brendza, A function for kinesin I in the posterior transport of oskar mRNA and Staufen protein. 2000, Pubmed
Bubunenko, A consensus RNA signal that directs germ layer determinants to the vegetal cortex of Xenopus oocytes. 2002, Pubmed , Xenbase
Bullock, The Drosophila hairy RNA localization signal modulates the kinetics of cytoplasmic mRNA transport. 2003, Pubmed
Chang, Localization of RNAs to the mitochondrial cloud in Xenopus oocytes through entrapment and association with endoplasmic reticulum. 2004, Pubmed , Xenbase
Chartrand, Asymmetric sorting of ash1p in yeast results from inhibition of translation by localization elements in the mRNA. 2002, Pubmed
Chartrand, Structural elements required for the localization of ASH1 mRNA and of a green fluorescent protein reporter particle in vivo. 1999, Pubmed
Chekulaeva, Bruno acts as a dual repressor of oskar translation, promoting mRNA oligomerization and formation of silencing particles. 2006, Pubmed
Chung, Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae. 2010, Pubmed
Cole, Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. 1993, Pubmed , Xenbase
Colegrove-Otero, The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis. 2005, Pubmed , Xenbase
Cote, A Xenopus protein related to hnRNP I has a role in cytoplasmic RNA localization. 1999, Pubmed , Xenbase
Dale, Secretion and mesoderm-inducing activity of the TGF-beta-related domain of Xenopus Vg1. 1993, Pubmed , Xenbase
Davis, Apical localization of pair-rule transcripts requires 3' sequences and limits protein diffusion in the Drosophila blastoderm embryo. 1991, Pubmed
Deng, Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation. 2008, Pubmed
Deshler, Localization of Xenopus Vg1 mRNA by Vera protein and the endoplasmic reticulum. 1997, Pubmed , Xenbase
Deshler, A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates. 1998, Pubmed , Xenbase
Dictenberg, A direct role for FMRP in activity-dependent dendritic mRNA transport links filopodial-spine morphogenesis to fragile X syndrome. 2008, Pubmed
Ding, Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis. 1993, Pubmed
Di Talia, Daughter-specific transcription factors regulate cell size control in budding yeast. 2009, Pubmed
Donnelly, Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity. 2011, Pubmed
dos Santos, A stem-loop structure in the wingless transcript defines a consensus motif for apical RNA transport. 2008, Pubmed
Doyle, Mechanisms of dendritic mRNA transport and its role in synaptic tagging. 2011, Pubmed
Du, Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA. 2008, Pubmed
Elvira, Characterization of an RNA granule from developing brain. 2006, Pubmed
Ephrussi, Induction of germ cell formation by oskar. 1992, Pubmed
Ephrussi, Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. 1991, Pubmed
Farina, Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment. 2003, Pubmed
Gonsalvez, ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex. 2004, Pubmed
Gonzalez, ASH1 mRNA localization in yeast involves multiple secondary structural elements and Ash1 protein translation. 1999, Pubmed
Gu, A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. 2004, Pubmed
Haarer, Identification of MYO4, a second class V myosin gene in yeast. 1994, Pubmed
Hachet, Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport. 2001, Pubmed
Hachet, Splicing of oskar RNA in the nucleus is coupled to its cytoplasmic localization. 2004, Pubmed
Havin, RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization. 1998, Pubmed , Xenbase
Hirokawa, Kinesin superfamily motor proteins and intracellular transport. 2009, Pubmed
Hüttelmaier, Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1. 2005, Pubmed
Jansen, Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins. 1996, Pubmed
Joseph, Mutant Vg1 ligands disrupt endoderm and mesoderm formation in Xenopus embryos. 1998, Pubmed , Xenbase
Kessler, Induction of dorsal mesoderm by soluble, mature Vg1 protein. 1995, Pubmed , Xenbase
Kim-Ha, oskar mRNA is localized to the posterior pole of the Drosophila oocyte. 1991, Pubmed
Kim-Ha, Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential. 1995, Pubmed
Kim-Ha, Multiple RNA regulatory elements mediate distinct steps in localization of oskar mRNA. 1993, Pubmed
Kislauskis, Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments. 1993, Pubmed
Kislauskis, Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype. 1994, Pubmed
Kraut-Cohen, Addressing mRNAs to the ER: cis sequences act up! 2010, Pubmed
Krementsova, Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex. 2011, Pubmed
Kress, Nuclear RNP complex assembly initiates cytoplasmic RNA localization. 2004, Pubmed , Xenbase
Kugler, Localization, anchoring and translational control of oskar, gurken, bicoid and nanos mRNA during Drosophila oogenesis. 2009, Pubmed
Kwon, UUCAC- and vera-dependent localization of VegT RNA in Xenopus oocytes. 2002, Pubmed , Xenbase
Landers, She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae. 2009, Pubmed
Lécuyer, Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function. 2007, Pubmed
Leung, Asymmetrical beta-actin mRNA translation in growth cones mediates attractive turning to netrin-1. 2006, Pubmed , Xenbase
Lewis, Conserved and clustered RNA recognition sequences are a critical feature of signals directing RNA localization in Xenopus oocytes. 2004, Pubmed , Xenbase
Lifland, Dynamics of native β-actin mRNA transport in the cytoplasm. 2011, Pubmed
Lionnet, A transgenic mouse for in vivo detection of endogenous labeled mRNA. 2011, Pubmed
Long, She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA. 2000, Pubmed
Long, Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. 1997, Pubmed
Mallardo, Isolation and characterization of Staufen-containing ribonucleoprotein particles from rat brain. 2003, Pubmed
Margeot, Why are many mRNAs translated to the vicinity of mitochondria: a role in protein complex assembly? 2005, Pubmed
Maxon, Ash1p is a site-specific DNA-binding protein that actively represses transcription. 2001, Pubmed
Messitt, Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes. 2008, Pubmed , Xenbase
Micklem, The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila. 1997, Pubmed
Mohr, The RNA-binding protein Tsunagi interacts with Mago Nashi to establish polarity and localize oskar mRNA during Drosophila oogenesis. 2001, Pubmed
Müller, A cytoplasmic complex mediates specific mRNA recognition and localization in yeast. 2011, Pubmed
Müller, Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization. 2009, Pubmed
Münchow, Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins. 1999, Pubmed
Nakamura, Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. 2004, Pubmed
Neuman-Silberberg, The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGF alpha-like protein. 1993, Pubmed
Newmark, mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila. 1997, Pubmed , Xenbase
Niessing, She2p is a novel RNA binding protein with a basic helical hairpin motif. 2004, Pubmed
Oeffinger, Comprehensive analysis of diverse ribonucleoprotein complexes. 2007, Pubmed
Otero, A 250-nucleotide UA-rich element in the 3' untranslated region of Xenopus laevis Vg1 mRNA represses translation both in vivo and in vitro. 2001, Pubmed , Xenbase
Palacios, An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay. 2004, Pubmed
Palacios, How does an mRNA find its way? Intracellular localisation of transcripts. 2007, Pubmed
Palacios, Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte. 2002, Pubmed
Pan, ZBP2 facilitates binding of ZBP1 to beta-actin mRNA during transcription. 2007, Pubmed
Paquin, Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p. 2007, Pubmed
Pfeiffer, Microtubules in Xenopus oocytes are oriented with their minus-ends towards the cortex. 1999, Pubmed , Xenbase
Reck-Peterson, The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. 2001, Pubmed
Reveal, BREs mediate both repression and activation of oskar mRNA translation and act in trans. 2010, Pubmed
Ross, Characterization of a beta-actin mRNA zipcode-binding protein. 1997, Pubmed
Sasaki, Phosphorylation of zipcode binding protein 1 is required for brain-derived neurotrophic factor signaling of local beta-actin synthesis and growth cone turning. 2010, Pubmed , Xenbase
Shen, Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p. 2009, Pubmed
Shen, Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. 2010, Pubmed
Shepard, Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. 2003, Pubmed
Sil, Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene. 1996, Pubmed
Simmonds, Apical localization of wingless transcripts is required for wingless signaling. 2001, Pubmed
Smith, Overexpression of oskar directs ectopic activation of nanos and presumptive pole cell formation in Drosophila embryos. 1992, Pubmed
St Johnston, Moving messages: the intracellular localization of mRNAs. 2005, Pubmed
St Johnston, Staufen, a gene required to localize maternal RNAs in the Drosophila egg. 1991, Pubmed
Takizawa, Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast. 1997, Pubmed
Takizawa, The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. 2000, Pubmed
Takizawa, Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier. 2000, Pubmed
Tang, A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites. 2001, Pubmed
Thomsen, Processed Vg1 protein is an axial mesoderm inducer in Xenopus. 1993, Pubmed , Xenbase
Tiruchinapalli, Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons. 2003, Pubmed
van Eeden, Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole. 2001, Pubmed
Weeks, A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta. 1987, Pubmed , Xenbase
Welshhans, Netrin-1-induced local β-actin synthesis and growth cone guidance requires zipcode binding protein 1. 2011, Pubmed
Wilhelm, Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. 2003, Pubmed
Wilhelm, Coordinate control of translation and localization of Vg1 mRNA in Xenopus oocytes. 2000, Pubmed , Xenbase
Wilhelm, Mechanisms of translational regulation in Drosophila. 2005, Pubmed
Yao, An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidance. 2006, Pubmed , Xenbase
Yisraeli, A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA. 1990, Pubmed , Xenbase
Yoon, Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin. 2004, Pubmed , Xenbase
Zhang, Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility. 2001, Pubmed
Zheng, A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons. 2001, Pubmed
Zimyanin, In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization. 2008, Pubmed