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Dev Cell 2008 Sep 01;153:426-436. doi: 10.1016/j.devcel.2008.06.014.
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Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.

Messitt TJ , Gagnon JA , Kreiling JA , Pratt CA , Yoon YJ , Mowry KL .

RNA localization is a widely conserved mechanism for generating cellular asymmetry. In Xenopus oocytes, microtubule-dependent transport of RNAs to the vegetal cortex underlies germ layer patterning. Although kinesin motors have been implicated in this process, the apparent polarity of the microtubule cytoskeleton has pointed instead to roles for minus-end-directed motors. To resolve this issue, we have analyzed participation of kinesin motors in vegetal RNA transport and identified a direct role for Xenopus kinesin-1. Moreover, in vivo interference and biochemical experiments reveal a key function for multiple motors, specifically kinesin-1 and kinesin-2, and suggest that these motors may interact during transport. Critically, we have discovered a subpopulation of microtubules with plus ends at the vegetal cortex, supporting roles for these kinesin motors in vegetal RNA transport. These results provide a new mechanistic basis for understanding directed RNA transport within the cytoplasm.

PubMed ID: 18771961
PMC ID: PMC2581415
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: gdf1 grap2 kif11 kif3b kif5b mapre1 psmd6 tuba4b tubg1
Antibodies: Kif1a Ab1 Kif5b Ab1 Mapre1 Ab1 Tuba4b Ab7

Article Images: [+] show captions
References [+] :
Alarcón, RNA anchoring in the vegetal cortex of the Xenopus oocyte. 2001, Pubmed, Xenbase